DECREASE IN NUCLEAR FEULGEN-POSITIVE MATERIAL (DNA) UPON AGING IN IN VITRO STORAGE OF BOVINE SPERMATOZOA | Zendy

G.W. Salisbury | Zendy; W.J. Birge | Zendy; Luis De La Torre | Zendy; J.R. Lodge | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

DECREASE IN NUCLEAR FEULGEN-POSITIVE MATERIAL (DNA) UPON AGING IN IN VITRO STORAGE OF BOVINE SPERMATOZOA

Author(s) -

G.W. Salisbury,

W.J. Birge,

Luis De La Torre,

J.R. Lodge

Publication year - 1961

Publication title -

the journal of cell biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.414

H-Index - 380

eISSN - 1540-8140

pISSN - 0021-9525

DOI - 10.1083/jcb.10.3.353

Subject(s) - feulgen stain , biology , andrology , sperm , dna , artificial insemination , insemination , semen , in vitro , biochemistry , anatomy , genetics , pregnancy , medicine

The Feulgen-DNA content of sperm cells from 5 bulls was studied by means of microspectrophotometry after storage at 5 degrees C for 2, 3, 5, and 10 days in a yolk-citrate diluent permitting slow aerobic metabolism. A subsample of sperm cells from each bull was subjected to the Feulgen technique on each of the storage days selected. The cells sampled on each of these days received a standard 12 minute, 60 degrees C hydrolysis. Absorption measurements at 546 mmicroof the individual cells indicated a marked progressive decrease in the Feulgen-DNA content of the stored spermatozoa. The loss of 30 per cent of the initial DNA at the end of 5 days' storage was highly significant statistically. This decrease approximately parallels the known decrease in fertility of stored sperm cells, as well as the increase in apparent embryonic mortality resulting from the use of similarly aged spermatozoa for artificial insemination.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research