BUFFERED OSMIUM TETROXIDE (OsO4) FIXATION FOR CYTOLOGICAL AND FEULGEN MICROSPECTROPHOTOMETRIC STUDIES OF HUMAN RECTAL POLYPS | Zendy

Cecilie Leuchtenberger | Zendy; Paul F. Doolin | Zendy; Anne H. Kutsakis | Zendy

Open Access

BUFFERED OSMIUM TETROXIDE (OsO4) FIXATION FOR CYTOLOGICAL AND FEULGEN MICROSPECTROPHOTOMETRIC STUDIES OF HUMAN RECTAL POLYPS

Author(s) -

Cecilie Leuchtenberger,

Paul F. Doolin,

Anne H. Kutsakis

Publication year - 1955

Publication title -

the journal of cell biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.414

H-Index - 380

eISSN - 1540-8140

pISSN - 0021-9525

DOI - 10.1083/jcb.1.5.385

Subject(s) - feulgen stain , osmium tetroxide , biology , fixation (population genetics) , staining , cytoplasm , pathology , chromatin , dna , osmium , microbiology and biotechnology , biochemistry , genetics , electron microscope , medicine , physics , gene , ruthenium , optics , catalysis

Evidence has been presented that when human rectal mucosa and polyps are fixed with buffered OsO(4) according to the method of Palade, staining of paraffin sections by basic and acidic dyes results in a distinct color difference between chromatin and cytoplasm. Furthermore, the Feulgen reaction also can be carried out successfully and such OsO(4)-fixed Feulgen-stained sections are especially suitable for microspectrophotometric determinations of DNA in individual nuclei.

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