Open AccessPostoperative exposure to glove powders modulates production of peritoneal eicosanoids during peritoneal wound healing
Author(s) -
Chegini Nasser,
Rong Hui
Publication year - 1999
Publication title -
european journal of surgery
Language(s) - English
Resource type - Journals
eISSN - 1741-9271
pISSN - 1102-4151
DOI - 10.1080/11024159950189771
Subject(s) - eicosanoid , medicine , peritoneal cavity , thromboxane b2 , peritoneal fluid , peritoneum , wound healing , thromboxane , saline , prostaglandin , lipopolysaccharide , thromboxane a2 , leukotriene , andrology , surgery , pathology , platelet , biochemistry , enzyme , chemistry , arachidonic acid , asthma
Objective: To assess the effect of postsurgical exposure of peritoneal cavity to glove powders, Hydrocote, latex proteins, and lipopolysaccharide (LPS) on eicosanoid production in peritoneal fluid and cellular distribution of eicosanoid enzymes in peritoneal wound during healing. Design: Randomised experimental study. Setting: Institute for Wound Research, USA. Animals: 360 mice randomised into six groups of 60 each. Intervention: Abrasion of peritoneal cavity followed by instillation of 500 μl of sterile phosphate buffered saline (PBS) alone (Control) or containing 100 μg/ml of Biosorb, Keoflo, Hydrocote, 1 mg/ml of latex proteins, or 12.5 μg/ml of LPS. Mice were killed at 1, 2, 4, 7, 14 and 28 days, and the peritoneal washing obtained from each animal and concentration of eicosanoids measured. Tissue were immunostained for cyclooxygenases and 5‐lipoxygenase and thromboxane A 2 (TXA 2 ) synthetase. Results: Peritoneal fluid from uninjured controls contained 3.9 ( 0.8), 5.2 ( 0.3) and 0.2 (0.02) ng/ml of thromboxane B 2 (TXB 2 ), prostaglandin E 2 (PGE 2 ) and leukotriene B 4 (LTB 4 ), respectively. These increased significantly during the first week to 6.3 (0.3), 11.7 (0.8) and 2.6 (0.1) ng/ml, p < 0.05, before returning to baseline by day 14. In all the treated groups the values were significantly higher than in controls ( p < 0.05). Imunoreactive cyclo‐oxygenases, 5‐lipoxygenase and TXA 2 synthetase proteins were present in various cell types in uninjured skin and peritoneum, incisional and peritoneal wounds and adhesion tissues. Staining was more intense at the site of wounds and paralleled eicosanoid concentrations during healing. There was no difference between exposed and unexposed groups. Conclusion: The presence of glove powders, latex proteins and LPS in peritoneal cavity cause increased eicosanoid production and aggravate the normal inflammatory reaction to tissue injury. This may contribute to the inflammatory or immune reactions and development of adhesions caused by glove powders. Copyright © 1999 Taylor and Francis Ltd.