Open AccessTreatment with hyperbaric oxygen affects endothelial cell fibrinolysis
Author(s) -
Tjärnström Johan,
Holmdahl Lena,
Arnell Per,
Falkenberg Mårten,
Risberg Bo
Publication year - 1999
Publication title -
european journal of surgery
Language(s) - English
Resource type - Journals
eISSN - 1741-9271
pISSN - 1102-4151
DOI - 10.1080/11024159950189302
Subject(s) - fibrinolysis , medicine , tissue plasminogen activator , hyperbaric oxygen , plasminogen activator , endothelial stem cell , room air distribution , plasminogen activator inhibitor 1 , surgery , andrology , anesthesia , immunology , chemistry , biochemistry , in vitro , physics , thermodynamics
Objective: To investigate the effect of hyperbaric oxygen treatment (HBO) on the thrombolytic properties of endothelial cells. Setting: University hospital, Sweden. Interventions: Human endothelial cells were derived from saphenous veins, and exposed to oxygen in a compression chamber at 2.5 atmospheres absolute (ATA, =250kPa). Cells exposed to 2.5 ATA with a gas mixture similar to air (HB Air), and unpressurised air‐exposed cells served as controls. Main outcome measures: Tissue‐type plasminogen activator (t‐PA) and plasminogen activator inhibitor type‐1 (PAI‐1). Results: Immediately after treatment there was a significant increase in t‐PA protein in the medium in cultures treated with HBO compared with HB Air ( p = 0.015, n = 6), and untreated controls ( p = 0.015, n = 6). The PAI‐1 concentration in media was also higher in the HBO‐treated group compared with HB Air ( p = 0.004, n = 6) and untreated controls ( p = 0.004, n = 6). Six hours after treatment there was still a significant increase in PAI‐1 in the HBO‐treated group compared with untreated controls ( p = 0.007, n = 6), but not with the pressure control. t‐PA concentrations were similar. Specific mRNA for t‐PA and PAI‐1 was detectable immediately after treatment and six hours later in all experimental groups as assessed by reverse transcriptase polymerase chain reaction (RT‐PCR). HBO increased the gene expression for both t‐PA and PAI‐1. Conclusions: HBO affects endothelial cell function and its fibrinolytic response. These findings may have clinical relevance in hyperbaric medicine and trauma care. Copyright © 1999 Taylor and Francis Ltd.