Open AccessMelatonin modulates mesenteric blood flow and TNFα concentrations after lipopolysaccharide challenge
Author(s) -
Baykal Atac,
Iskit Alper B.,
Hamaloglu Erhan,
Guc M. Oguz,
Hascelik Gulsen,
Sayek Iskender
Publication year - 2000
Publication title -
european journal of surgery
Language(s) - English
Resource type - Journals
eISSN - 1741-9271
pISSN - 1102-4151
DOI - 10.1080/110241500750008484
Subject(s) - melatonin , lipopolysaccharide , medicine , endocrinology , tumor necrosis factor alpha , saline , blood flow , necrosis
Objective: To investigate the effect of various doses of melatonin on reduction in mesenteric blood flow (MBF) and increase in tumour necrosis factor alpha (TNFα) concentration caused by injection of lipopolysaccharide (LPS). Design: University Hospital, Turkey. Setting: Open experimental study. Animals: 59 Swiss albino mice. Interventions: Animals were injected with melatonin solvent or 1, 10, 100, or 500 mg/kg melatonin. Ten minutes later control animals were injected with saline, and the experimental group with LPS. Main outcome measures: Mesenteric blood flow and serum TNFα concentration. Results: In control animals, 100 and 500 mg/kg melatonin reduced MBF. LPS reduced MBF in solvent, 1, and 10 mg/kg melatonin groups. The concentration of TNFα was considerably increased in the mice given LPS. Melatonin reduced this response significantly. Conclusion: In high doses melatonin directly reduces MBF. It has no protective effect on the LPS‐induced decrease in MBF. In lower doses it blocks, but at higher doses reduces, LPS‐induced TNFα production. Copyright © 2000 Taylor and Francis Ltd.