Open AccessHyaluronic acid/carboxymethylcellulose membrane surrounding an intraperitoneal or subcutaneous jejunojejunostomy in rats
Author(s) -
van Oosterom Frederik J. T.,
van Lanschot Jan J. B.,
Oosting Johannes,
Obertop Huug
Publication year - 2000
Publication title -
european journal of surgery
Language(s) - English
Resource type - Journals
eISSN - 1741-9271
pISSN - 1102-4151
DOI - 10.1080/110241500750008349
Subject(s) - medicine , anastomosis , hydroxyproline , hyaluronic acid , surgery , subcutaneous injection , laparoscopy , abdominal wall , anesthesia , anatomy
Objective: To investigate the healing of intra‐abdominal and extra‐abdominal jejunal anastomoses that were surrounded by a hyaluronic acid/carboxymethylcellulose membrane (Seprafilm ® ) in rats. Design: Laboratory study. Setting: University hospital, The Netherlands. Animals: 56 male Wistar rats. Interventions: 28 rats had jejunojejunostomies placed subcutaneously and 28 had them placed intra‐abdominally. Half of each group of anastomoses were surrounded by Seprafilm ® . Main outcome measures: After 3 or 7 days, the anastomoses were tested for bursting pressure, tensile strength, and hydroxyproline concentrations. Results: There were numerous strong adhesions around the anastomoses in the subcutaneous position and Seprafilm ® had no influence on their extent. Bursting pressure and hydroxyproline concentrations were not affected by position or Seprafilm ® treatment. Tensile strength was significantly higher in the subcutaneous position ( p < 0.01), but was unaffected by Seprafilm ® . Conclusion: Anastomotic healing was not impaired in the subcutaneous or intraperitoneal position in this model. Seprafilm ® had no effect on the anastomosis. Copyright © 2000 Taylor and Francis Ltd.