Open AccessAn immunoassay for the measurement of (1→3)‐β‐D‐glucans in the indoor environment
Author(s) -
Jeroen Douwes,
G. Doekes,
R.C. Montijn,
Dick Heederik,
Bert Brunekreef
Publication year - 1997
Publication title -
mediators of inflammation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.37
H-Index - 97
eISSN - 1466-1861
pISSN - 0962-9351
DOI - 10.1080/09629359791578
Subject(s) - laminarin , curdlan , immunoassay , glucan , polysaccharide , antibody , chemistry , calibration curve , chromatography , microbiology and biotechnology , food science , biology , detection limit , biochemistry , immunology
An inhibition enzyme immunoassay was developed for quantitation of (1-->3)-beta-D-glucans in the indoor environment. Immunospecific rabbit antibodies were produced by immunization with bovine serum albuminconjugated laminarin.The laminarin calibration curve ranged from 40 to 3000 ng/ml.Another (1-->3)-beta-D-glucan (curdlan) showed a similar inhibition curve, but was less reactive on a weight basis. Pustulan, presumed to be (1-->3)-beta-D-glucan, also showed immunoreactivity in the assay. Control experiments indicated that this was due to (1-->3)-beta-D-glucan structures. Other non-(1-->3)-beta-D-glucan polysaccharides did not react. (1-->3)-beta-Dglucan was detectable in dust from a variety of occupational and environmental settings. We conclude that the new assay offers a useful method for indoor (1-->3)-beta-Dglucan exposure assessment.
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