Expression of a MORN repeat protein from Euplotes octocarinatus requires a +1 programmed ribosomal frameshifting | Zendy

Lili Wei | Zendy; Xuemei Zhao | Zendy; Ruanlin Wang | Zendy; Yuejun Fu | Zendy; Baofeng Chai | Zendy; Aihua Liang | Zendy

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Expression of a MORN repeat protein from Euplotes octocarinatus requires a +1 programmed ribosomal frameshifting

Author(s) -

Lili Wei,

Xuemei Zhao,

Ruanlin Wang,

Yuejun Fu,

Baofeng Chai,

Aihua Liang

Publication year - 2017

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1080/09168451.2017.1301804

Subject(s) - translational frameshift , biology , genetics , microbiology and biotechnology , gene , mutation , frameshift mutation

Analysis of transcriptome revealed that a membrane occupation and recognition nexus (MORN) repeat protein-encoding gene of Euplotes octocarinatus (Eo-morn-9-31) was a candidate for programmed +1 ribosomal frameshifting (+1 PRF). In this study, a dual-luciferase assay was performed to detect its expression. The result showed that the MORN repeat protein (Eo-MORN-9-31) could be produced by the +1 PRF event during the process of translation in yeast and the frameshifting efficiency was about 4–5%. We further confirmed its reality by western blot and mass spectrometry. This study provided experimental evidence indicating that the expression of the Eo-MORN-9-31 of E. octocarinatus required the +1 PRF.

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