Rapid separation of seed gliadins by reversed-phase ultra performance liquid chromatography (RP-UPLC) and its application in wheat cultivar and germplasm identification | Zendy

Caixia Han | Zendy; Xaiobing Lu | Zendy; Zitong Yu | Zendy; Xiaohui Li | Zendy; Wujun Ma | Zendy; Yueming Yan | Zendy

Open Access

Rapid separation of seed gliadins by reversed-phase ultra performance liquid chromatography (RP-UPLC) and its application in wheat cultivar and germplasm identification

Author(s) -

Caixia Han,

Xaiobing Lu,

Zitong Yu,

Xiaohui Li,

Wujun Ma,

Yueming Yan

Publication year - 2015

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1080/09168451.2014.998618

Subject(s) - germplasm , chromatography , high performance liquid chromatography , cultivar , gliadin , chemistry , identification (biology) , glutenin , gluten , agronomy , biology , botany , food science , biochemistry , gene , protein subunit

To separate gliadin from wheat flour, a novel and stability-indicating reversed-phase ultra performance liquid chromatography (RP-UPLC) method is established and optimized. A comparative analysis of routine capillary electrophoresis (CE), reversed-phase high-performance liquid chromatography (RP-HPLC), and RP-UPLC was performed and the results showed that the resolution and efficiency of RP-UPLC were significantly higher than those of CE and RP-HPLC. Characteristic RP-UPLC patterns of different bread wheat variety and related species were readily identified. These results demonstrated that our RP-UPLC procedure resulted in significant improvements in sensitivity, speed, and resolution, and thus is highly useful in wheat cultivar and germplasm identification.

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