Open AccessSAC3 may link nuclear protein export to cell cycle progression
Author(s) -
Amy Jones,
Brooks Quimby,
Jennifer K. Hood,
Paul Ko Ferrigno,
Praveen H. Keshava,
Pamela A. Silver,
Anita H. Corbett
Publication year - 2000
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.97.7.3224
Subject(s) - nuclear export signal , nucleoporin , biology , nuclear transport , nuclear protein , nuclear pore , cell nucleus , microbiology and biotechnology , mitosis , cytoplasm , cell cycle , nuclear localization sequence , karyopherin , cell cycle protein , ran , genetics , cell , gene , transcription factor
Selective movement of proteins between the nucleus and the cytoplasm is a regulatory mechanism exploited extensively by the eukaryotic cell. We have identified the evolutionarily conserved Sac3 protein, which was implicated previously in the regulation of mitosis [Bauer, A. & Kölling, R. (1996)J. Cell Sci. 109, 1575–1583] as a novel mediator of nuclear protein export. We show that Sac3p is localized to the nuclear pore, where it interacts with nucleoporins. Loss ofSAC3 function results in a block in nuclear export of a nuclear export signal-containing reporter protein. Our results also demonstrate thatSAC3 interacts genetically with the nuclear protein export factors Crm1p/Xpo1p and Yrb2p. Taken together, these data indicate a link between nuclear protein export and transition through the cell cycle.
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