ATM is a cytoplasmic protein in mouse brain required to prevent lysosomal accumulation

We previously generated a mouse model with a mutation in the murineAtm gene that recapitulates many aspects of the childhood neurodegenerative disease ataxia-telangiectasia.Atm -deficient (Atm −/−) mice show neurological defects detected by motor function tests including the rota-rod, open-field tests and hind-paw footprint analysis. However, no gross histological abnormalities have been observed consistently in the cerebellum of any line ofAtm −/− mice analyzed in most laboratories. Therefore, it may be that the neurologic dysfunction found in these animals is associated with predegenerative lesions. We performed a detailed analysis of the cerebellar morphology in two independently generated lines ofAtm −/− mice to determine whether there was evidence of neuronal abnormality. We found a significant increase in the number of lysosomes inAtm −/− mice in the absence of any detectable signs of neuronal degeneration or other ultrastructural anomalies. In addition, we found that the ATM protein is predominantly cytoplasmic in Purkinje cells and other neurons, in contrast to the nuclear localization of ATM protein observed in cultured cells. The cytoplasmic localization of ATM in Purkinje cells is similar to that found in human cerebellum. These findings suggest that ATM may be important as a cytoplasmic protein in neurons and that its absence leads to abnormalities of cytoplasmic organelles reflected as an increase in lysosomal numbers.