Open AccessA model for studying megakaryocyte development and biology
Author(s) -
George J. Murphy,
Andrew D. Leavitt
Publication year - 1999
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.96.6.3065
Subject(s) - megakaryocyte , lineage (genetic) , biology , progenitor cell , transgene , genetically modified mouse , microbiology and biotechnology , population , progenitor , gene , genetics , computational biology , stem cell , medicine , environmental health
The limited current understanding of megakaryocyte-lineage development and megakaryocyte biology is in large part because of a paucity of useful systems in which to conduct experiments. To overcome this problem, we have developed a transgenic mouse that uses theGP-Ib α regulatory sequences to achieve megakaryocyte-lineage restricted expression of an avian retroviral receptor. Through the transgenic avian receptor, avian retroviruses can efficiently and selectively infect megakaryocyte-lineage cellsin vitro andin vivo . Serial infections can be performed to introduce and express multiple genes in the same cell. We have used this system to generate and characterize a pure population of primary CD41-positive megakaryocyte progenitors.