Open AccessGenomic analysis of human and mouse TCL1 loci reveals a complex of tightly clustered genes
Author(s) -
Cora Hallas,
Yuri Pekarsky,
Takahiro Itoyama,
James M. Varnum,
Roberta Bichi,
Jay L. Rothstein,
Carlo M. Croce
Publication year - 1999
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.96.25.14418
Subject(s) - gene , biology , locus (genetics) , genetics , homology (biology) , homologous chromosome , gene knockin , chromosomal translocation , microbiology and biotechnology
TCL1 andTCL1b genes on human chromosome 14q23.1 are activated in T cell leukemias by translocations and inversions at 14q32.1, juxtaposing them to regulatory elements of T cell receptor genes. In this report we present the cloning, mapping, and expression analysis of the human and murineTCL1/Tcl1 locus. In addition toTCL1 andTCL1b , the human locus contains two additional genes,TCL1 -neighboring genes (TNG ) 1 and 2, encoding proteins of 141 and 110 aa, respectively. Both genes show no homology to any known genes, but their expression profiles are very similar to those ofTCL1 andTCL1b. TNG1 andTNG2 also are activated in T cell leukemias with rearrangements at 14q32.1. To aid in the development of a mouse model we also have characterized the murineTcl1 locus and found five genes homologous to humanTCL1b. Tcl1b1 –Tcl1b5 proteins range from 117 to 123 aa and are 65–80% similar, but they show only a 30–40% similarity to humanTCL1b . All five mouseTcl1b and murineTcl1 mRNAs are abundant in mouse oocytes and two-cell embryos but rare in various adult tissues and lymphoid cell lines. These data suggest a similar or complementary function of these proteins in early embryogenesis.