Open AccessAccumulation of specific RNAs encoding transcriptional factors and stress response proteins against a background of severe depletion of cellular RNAs in cells infected with herpes simplex virus 1
Author(s) -
Nikolai N. Khodarev,
Sunil J. Advani,
Nalin Gupta,
Bernard Roizman,
Ralph R. Weichselbaum
Publication year - 1999
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.96.21.12062
Subject(s) - biology , herpes simplex virus , gene , virus , rna , gene expression , cell culture , virology , messenger rna , regulation of gene expression , microbiology and biotechnology , genetics
Herpes simplex virus 1 encodes several functions to preclude the shutoff of host response to infection, including degradation of mRNA immediately after infection. To determine whether any cellular mRNAs accumulate in infected cells against a background of severe loss of host RNA, we hybridized cDNAs derived from three different cell lines infected with wild type and a mutant virus to a DNA array containing probes for 588 human genes representing different functional groups. The results were that (i ) infected cells accumulated at levels above those of mock-infected cells, a small number of transcripts representing transcriptional factors that could regulate gene expression both positively and negatively, and one stress response protein (GADD45), (ii ) the amount and nature of the accumulated transcripts showed limited variability depending on the cell and virus, and (iii ) at least some of the proteins encoded by the accumulated transcripts could benefit either the virus or the host.