Influence of drinking water and diet on the stable-hydrogen isotope ratios of animal tissues

Despite considerable interest in using stable-hydrogen isotope ratio (deltaD) measurements in ecological research, it was previously unknown whether hydrogen derived from drinking water, in addition to that derived from diet, contributed to the nonexchangeable hydrogen in animal tissues. We raised four experimental groups of quail (Coturnix coturnix japonica) from hatch on two isotopically distinct diets (mean nonexchangeable deltaD: -146 and -60 per thousand, Vienna Standard Mean Ocean Water Standard) and drinking waters (mean deltaD: -130 and +196 per thousand, Vienna Standard Mean Ocean Water Standard). Here we show that both dietary and drinking water hydrogen are incorporated into nonexchangeable hydrogen in both metabolically active (i.e., muscle, liver, blood, fat) and inactive (i.e., feather, nail) tissues. Approximately 20% of hydrogen in metabolically active quail tissues and 26-32% of feathers and nail was derived from drinking water. Our findings suggest environmental interpretations of deltaD values from modern and fossil animal tissues may need to account for potentially large isotopic differences between drinking water and food and require a good understanding of the physiological ecology of study organisms.