Mediator protein mutations that selectively abolish activated transcription | Zendy

Lawrence C. Myers | Zendy; Claes M. Gustafsson | Zendy; Kathleen Hayashibara | Zendy; Patrick O. Brown | Zendy; Roger D. Kornberg | Zendy

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Mediator protein mutations that selectively abolish activated transcription

Author(s) -

Lawrence C. Myers,

Claes M. Gustafsson,

Kathleen Hayashibara,

Patrick O. Brown,

Roger D. Kornberg

Publication year - 1999

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.96.1.67

Subject(s) - mediator , transcription factor ii h , general transcription factor , coactivator , biology , transcription (linguistics) , rna polymerase ii , microbiology and biotechnology , upstream activating sequence , transcription factor , genetics , gene , enhancer , gene expression , promoter , linguistics , philosophy

Deletion of any one of three subunits of the yeast Mediator of transcriptional regulation, Med2, Pgd1 (Hrs1), and Sin4, abolished activation by Gal4-VP16 in vitro. By contrast, other Mediator functions, stimulation of basal transcription and of TFIIH kinase activity, were unaffected. A different but overlapping Mediator subunit dependence was found for activation by Gcn4. The genetic requirements for activation in vivo were closely coincident with those in vitro. A whole genome expression profile of a Deltamed2 strain showed diminished transcription of a subset of inducible genes but only minor effects on "basal" transcription. These findings make an important connection between transcriptional activation in vitro and in vivo, and identify Mediator as a "global" transcriptional coactivator.

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