Open AccessBacteria-induced neo-biosynthesis, stabilization, and surface expression of functional class I molecules in mouse dendritic cells
Author(s) -
María Rescigno,
Stefania Citterio,
Clotilde Théry,
Michael Rittig,
Donata Medaglini,
Gianni Pozzi,
Sébastian Amigorena,
Paola RicciardiCastagnoli
Publication year - 1998
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.95.9.5229
Subject(s) - mhc class i , antigen presentation , transporter associated with antigen processing , antigen processing , cd74 , cd1 , mhc class ii , biology , microbiology and biotechnology , major histocompatibility complex , antigen , phagolysosome , mhc restriction , antigen presenting cell , t cell , immunology , immune system , phagocytosis , phagosome
Here, we show that bacteria induce de novo synthesis of both major histocompatability complex (MHC) class I and II molecules in a mouse dendritic cell culture system. The neo-biosynthesis of MHC class I molecules is delayed as compared with that of MHC class II. Furthermore, bacteria stabilize MHC class I molecules by a 3-fold increase of their half-life. This has important consequences for the capacity of dendritic cells to present bacterial antigens in the draining lymph nodes. In addition, a model antigen, ovalbumin, expressed on the surface of recombinant Streptococcus gordonii is processed and presented on MHC class I molecules. This presentation is 10(6) times more efficient than that of soluble OVA protein. This exogenous pathway of MHC class I presentation is transporter associated with antigen processing (TAP)-dependent, indicating that there is a transport from phagolysosome to cytosol in dendritic cells. Thus, bacteria are shown to be a potentially useful mean for the correct delivery of exogenous antigens to be presented efficiently on MHC class I molecules.