Open AccessA mAb recognizing a surface antigen ofMycobacterium tuberculosisenhances host survival
Author(s) -
Rachel Teitelbaum,
Aharona GlatmanFreedman,
Bing Chen,
John B. Robbins,
Emil R. Unanue,
Arturo Casadevall,
Barry R. Bloom
Publication year - 1998
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.95.26.15688
Subject(s) - mycobacterium tuberculosis , monoclonal antibody , tuberculosis , antigen , microbiology and biotechnology , biology , immune system , virulence , immunology , major histocompatibility complex , virology , epitope , interferon gamma , pathogen , antibody , gene , medicine , pathology , biochemistry
Murine mAbs reactive with the surface ofMycobacterium tuberculosis were assayed for their ability to affect the course of infection in mice challenged with virulent organisms. An IgG3 mAb (9d8) specific for arabinomannan and reactive with purified antigen from a clinical isolate ofM. tuberculosis conferred partial protection on mice after respiratory challenge (30–60% survival >75 days;P ≤ 0.05). Control mice pretreated with an irrelevant mAb of the same isotype succumbed to tuberculosis within 30 days. Mice with gene disruptions in interferon γ and major histocompatibility complex Class II also were partially protected from challenge. The protective mAb was neither bactericidal nor inhibitory of infection or bacterial replication. Nevertheless, it profoundly altered the nature of the granulomas in the infected lungs. Mice treated with mAb 9d8 and challenged withM. tuberculosis localized the pathogen within granuloma centers, suggesting that the mAb conferred protection by enhancing a cellular immune response.