Open AccessDopamine-induced recruitment of dopamine D1 receptors to the plasma membrane
Author(s) -
Hjalmar Brismar,
Mohammad Asghar,
Robert M. Carey,
Paul Greengard,
Anita Aperia
Publication year - 1998
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.95.10.5573
Subject(s) - dopamine , receptor , microbiology and biotechnology , dopamine receptor , cell fractionation , cytoplasm , d1 like receptor , dopamine receptor d1 , cytosol , d2 like receptor , endogenous agonist , agonist , chemistry , biology , endocrinology , membrane , biochemistry , enzyme
The recruitment of G protein-coupled receptors from the cytoplasm to the plasma membrane generally is believed to be a constitutive process. We show here by the use of both confocal microscopy and subcellular fractionation that, for at least one such receptor, this recruitment is regulated and not constitutive. Cells from a proximal tubular-like cell line, LLCPK1 cells, were incubated with either a D1 agonist, a dopamine precursor, or an inhibitor of dopamine metabolism to increase dopamine availability in the cell. Each of the three procedures led to a rapid translocation of dopamine D1 receptors from the cytosol to the plasma membrane.