Open AccessA putative angiogenin receptor in angiogenin-responsive human endothelial cells
Author(s) -
Guofu Hu,
James Riordan,
Bert L. Vallée
Publication year - 1997
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.94.6.2204
Subject(s) - angiogenin , angiogenesis , monoclonal antibody , receptor , microbiology and biotechnology , biology , rnase p , cell growth , endothelial stem cell , antibody , chemistry , biochemistry , cancer research , immunology , in vitro , rna , gene
Angiogenin stimulates both [3 H]thymidine incorporation and proliferation of human endothelial cells in sparse cultures. Under these conditions, a 170-kDa cell surface protein can be detected that binds angiogenin specifically. Angiogenin-stimulated cell growth is concentration-dependent and is completely inhibited by an anti-angiogenin monoclonal antibody, but not by a nonimmune control antibody. It is not affected by the nonangiogenic homolog, RNase A, nor by other angiogenic proteins, such as basic fibroblast growth factor and its antibody. Results suggest that under specific conditions, endothelial cells express an angiogenin receptor that may mediate angiogenin-stimulated DNA synthesis and proliferation and play an important role in angiogenin-induced angiogenesis.