Open AccessHighly purified CD25−resting T cells cannot be infectedde novowith HIV-1
Author(s) -
Chin-Sheng Chou,
Octavio Ramilo,
Ellen S. Vitetta
Publication year - 1997
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.94.4.1361
Subject(s) - il 2 receptor , peripheral blood mononuclear cell , biology , virology , microbiology and biotechnology , in vitro , cytotoxic t cell , genetics
Previous studies have demonstrated that the expression of CD25 can distinguish CD25− latently infected cells from CD25+ cells actively producing virus. Our studies were designed to characterize the nature and stability of the viral genome in CD25− quiescent HIV-1-infected cells and to determine whether these cells could be infectedde novo with HIV-1. Our results show that: (i ) When unfractionated peripheral blood mononuclear cells are first infected with HIV-1 and the CD25− cells then isolated, the latter contain only incomplete DNA transcripts and no full-length DNA or 2-LTR circles. Phytohemagglutinin activation of these CD25− cells results in the generation of full-length viral DNA and p24 production. (ii ) When CD25− CD4+ cells are first purified from peripheral blood mononuclear cells and then incubated with HIV-1, viral DNA cannot be detected, suggesting that these purified cells cannot be infected. Furthermore, CD25− adherent cells do not facilitate the infection of CD4+ CD25− T cells when they were present at the time of incubation with HIV-1. Taken together, these studies suggest either that (i ) the CD25− cells containing incomplete DNA transcripts are derived from infected-activated CD25+ cells, which subsequently become CD25− or (ii ) the presence of CD25+ cells is required for the infection of CD25− cellsin vitro .