Site-directed mutations reveal long-range compensatory interactions in the Adh gene of Drosophila melanogaster

Long-range interactions between the 5′ and 3′ ends of mRNA molecules have been suggested to play a role in the initiation of translation and the regulation of gene expression. To identify such interactions and to study their molecular evolution, we used phylogenetic analysis to generate a model of mRNA higher-order structure in theAdh transcript ofDrosophila melanogaster . This model predicts long-range, tertiary contacts between a region of the protein-encoding sequence just downstream of the start codon and a conserved sequence in the 3′ untranslated region (UTR). To further examine the proposed structure, site-directed mutations were generatedin vitro in a clonedD. melanogaster Adh gene, and the mutant constructs were introduced into theDrosophila germ line through P-element mediated transformation. Transformants were spectrophotometrically assayed for alcohol dehydrogenase activity. Our results indicate that transformants containing a silent mutation near the start of the protein-encoding sequence show an ≈15% reduction in alcohol dehydrogenase activity relative to wild-type transformants. This activity can be restored to wild-type levels by a second, compensatory mutation in the 3′ UTR. These observations are consistent with a higher-order structure model that includes long-range interactions between the 5′ and 3′ ends of theAdh mRNA. However, our results do not fit the classical compensatory substitution model because the second mutation by itself (in the 3′ UTR) did not show a measurable reduction in gene expression.