Open AccessMitosis-promoting factor-mediated suppression of a cloned delayed rectifier potassium channel expressed in Xenopus oocytes
Author(s) -
Andrea Brüggemann,
Walter Stühmer,
Luis A. Pardo
Publication year - 1997
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.94.2.537
Subject(s) - xenopus , mitosis , microbiology and biotechnology , oocyte , maturation promoting factor , potassium channel , cell cycle , biology , inward rectifier potassium ion channel , cyclin dependent kinase 1 , cyclin b , cyclin , chemistry , ion channel , genetics , biophysics , cell , gene , embryo , receptor
The cell cycle is the crucial process that leads to mitosis in all cell types. The dramatic redirectioning of many cellular processes during the cycle is known to involve ion channels, either changing their level of expression or their voltage dependence, as in the case of inward rectifiers. Here we describe the specific inhibition of heterologously expressed ionic channels at the onset of maturation inXenopus oocytes. In cells expressing rateag (R-eag ) potassium channels, maturation induces a dramatic reduction in the current amplitude, which is almost complete in most cases. The key molecule in oocyte maturation, the mitosis-promoting factor (a complex of cyclin B and p34cdc2 ), is able to induce similar changes when injected into the oocytes.