Open AccessCardioprotective effects of 70-kDa heat shock protein in transgenic mice.
Author(s) -
Nina B. Radford,
Maggy Fina,
Ivor J. Benjamin,
Randall W. Moreadith,
Kathy H. Graves,
Piyu Zhao,
Sandya R. Gavva,
Andrea J. Wiethoff,
A. Dean Sherry,
Craig R. Malloy,
R. Sanders Williams
Publication year - 1996
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.93.6.2339
Subject(s) - heat shock protein , shock (circulatory) , metabolic acidosis , genetically modified mouse , transgene , hsp70 , biology , microbiology and biotechnology , acidosis , biochemistry , chemistry , medicine , endocrinology , gene
Heat shock proteins are proposed to limit injury resulting from diverse environmental stresses, but direct metabolic evidence for such a cytoprotective function in vertebrates has been largely limited to studies of cultured cells. We generated lines of transgenic mice to express human 70-kDa heat shock protein constitutively in the myocardium. Hearts isolated from these animals demonstrated enhanced recovery of high energy phosphate stores and correction of metabolic acidosis following brief periods of global ischemia sufficient to induce sustained abnormalities of these variables in hearts from nontransgenic littermates. These data demonstrate a direct cardioprotective effect of 70-kDa heat shock protein to enhance postischemic recovery of the intact heart.
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