Open AccessOn the molecular basis and regulation of cellular capacitative calcium entry: Roles for Trp proteins
Author(s) -
Lutz Birnbaumer,
Xi Zhu,
Meisheng Jiang,
Guylain Boulay,
Michael Peyton,
Brigitte Vannier,
Darren M. Brown,
Daniela Platano,
Hamid Mirmohammad Sadeghi,
Enrico Stefani,
Mariel Birnbaumer
Publication year - 1996
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.93.26.15195
Subject(s) - gene , biology , intracellular , microbiology and biotechnology , endogeny , calcium in biology , gene expression , calcium , calcium signaling , homeostasis , genetics , biochemistry , medicine
During the last 2 years, our laboratory has worked on the elucidation of the molecular basis of capacitative calcium entry (CCE) into cells. Specifically, we tested the hypothesis that CCE channels are formed of subunits encoded in genes related to the Drosophilatrp gene. The first step in this pursuit was to search for mammaliantrp genes. We found not one but six mammalian genes and cloned several of their cDNAs, some in their full length. As assayed in mammalian cells, overexpression of some mammalian Trps increases CCE, while expression of partialtrp cDNAs in antisense orientation can interfere with endogenous CCE. These findings provided a firm connection between CCE and mammalian Trps. This article reviews the known forms of CCE and highlights unanswered questions in our understanding of intracellular Ca2+ homeostasis and the physiological roles of CCE.