Open AccessHDA1 and RPD3 are members of distinct yeast histone deacetylase complexes that regulate silencing and transcription
Author(s) -
Stephen E. Rundlett,
Andrew A. Carmen,
Ryûji Kobayashi,
Sergei G. Bavykin,
Bryan M. Turner,
Michael Grunstein
Publication year - 1996
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.93.25.14503
Subject(s) - histone deacetylase 5 , histone deacetylase , sap30 , biology , hdac4 , hdac11 , histone h2a , histone h4 , trichostatin a , acetylation , histone , microbiology and biotechnology , genetics , gene
Increased histone acetylation has been correlated with increased transcription, and regions of heterochromatin are generally hypoacetylated. In investigating the cause-and-effect relationship between histone acetylation and gene activity, we have characterized two yeast histone deacetylase complexes. Histone deacetylase-A (HDA) is an ≈350-kDa complex that is highly sensitive to the deacetylase inhibitor trichostatin A. Histone deacetylase-B (HDB) is an ≈600-kDa complex that is much less sensitive to trichostatin A. The HDA1 protein (a subunit of the HDA activity) shares sequence similarity to RPD3, a factor required for optimal transcription of certain yeast genes. RPD3 is associated with the HDB activity.HDA1 also shares similarity to three new open reading frames in yeast, designatedHOS1 ,HOS2 , andHOS3 . We find that bothhda1 andrpd3 deletions increase acetylation levelsin vivo at all sites examined in both core histones H3 and H4, withrpd3 deletions having a greater impact on histone H4 lysine positions 5 and 12. Surprisingly, bothhda1 andrpd3 deletions increase repression at telomeric loci, which resemble heterochromatin withrpd3 having a greater effect. In addition,rpd3 deletions retard full induction of thePHO5 promoter fused to the reporterlacZ . These data demonstrate that histone acetylation state has a role in regulating both heterochromatic silencing and regulated gene expression.