Open AccessBrca2 is coordinately regulated with Brca1 during proliferation and differentiation in mammary epithelial cells
Author(s) -
Jayant V. Rajan,
Man Wang,
Sandra T. Marquis,
Lewis A. Chodosh
Publication year - 1996
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.93.23.13078
Subject(s) - microbiology and biotechnology , cell growth , mammary gland , cellular differentiation , biology , cancer research , chemistry , genetics , breast cancer , gene , cancer
We have analyzed the expression of the breast cancer susceptibility gene,Brca2 , in mammary epithelial cells as a function of proliferation and differentiation. Our results demonstrate thatBrca2 mRNA expression is tightly regulated during mammary epithelial proliferation and differentiation, and that this regulation occurs coordinately withBrca1 . Specifically,Brca2 mRNA expression is up-regulated in rapidly proliferating cells; is down-regulated in response to serum deprivation; is expressed in a cell cycle-dependent manner, peaking at the G1 /S boundary; and is up-regulated in differentiating mammary epithelial cells in response to glucocorticoids. In each case, an identical pattern of expression was observed forBrca1 . These results indicate that proliferative stimuli modulate the mRNA expression of these two breast cancer susceptibility genes. In addition, the coordinate regulation ofBrca1 andBrca2 revealed by these experiments suggests that these genes are induced by, and may function in, overlapping regulatory pathways involved in the control of cell proliferation and differentiation.