Open AccessApplication of capillary electrophoresis-electrospray ionization mass spectometry in the determination of molecular diversity.
Author(s) -
Yuriy M. Dunayevskiy,
Paul Vouros,
Edward A. Wintner,
Gerald W. Shipps,
Thomas Carell,
Julius Rebek
Publication year - 1996
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.93.12.6152
Subject(s) - capillary electrophoresis , chemistry , xanthene , chromatography , electrospray ionization , electrospray , mass spectrometry , capillary electrophoresis–mass spectrometry , electrophoresis , analyte , analytical chemistry (journal) , organic chemistry
By means of capillary electrophoresis coupled online to electrospray ionization MS, a library of theoretically 171 disubstituted xanthene derivatives was analyzed. The method allowed the purity and makeup of the library to be determined: 160 of the expected compounds were found to be present, and 12 side-products were also detected in the mixture. Due to the ability of capillary electrophoresis to separate analytes on the basis of charge, most of the xanthene derivatives could be resolved by simple capillary electrophoresis-MS procedures even though 124 of the 171 theoretical compounds were isobaric with at least one other molecule in the mixture. Any remaining unresolved peaks were resolved by MS/MS experiments. The method shows promise for the analysis of small combinatorial libraries with fewer than 1000 components.