Open AccessMos overexpression in Swiss 3T3 cells induces meiotic-like alterations of the mitotic spindle.
Author(s) -
Kenji Fukasawa,
George F. Vande Woude
Publication year - 1995
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.92.8.3430
Subject(s) - cytokinesis , microbiology and biotechnology , mitosis , multipolar spindles , binucleated cells , spindle apparatus , spindle pole body , meiosis , biology , polar body , oocyte , somatic cell , spindle checkpoint , chemistry , cell division , cell , genetics , embryo , gene , micronucleus , micronucleus test , organic chemistry , toxicity
High levels of mos protooncogene product are expressed during oocyte meiotic maturation and Mos has been implicated in formation of the spindle and spindle pole. Here, we show that in Swiss 3T3 cells with 4N DNA content, high levels of Mos lead to the production of binucleated cells. The Swiss 3T3 cells in mitosis, before binucleation occurs, are anastral and the spindle poles are juxtaposed to the cell membrane. These phenotypes may be related to the meiotic process of attachment of the spindle pole to the oocyte membrane during polar body formation. The production of binucleated somatic cells could result from attachment of the altered mitotic spindle pole to the cell membrane that interferes with cytokinesis but not karyokinesis. This can explain at least one form of genetic instability that leads to altered DNA content in tumor cells.
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