Open AccessP-Lim, a LIM homeodomain factor, is expressed during pituitary organ and cell commitment and synergizes with Pit-1.
Author(s) -
Ingolf Bach,
Simon J. Rhodes,
Richard V. Pearse,
Thorsten Heinzel,
Bernd Gloss,
Kathleen M. Scully,
Paul Sawchenko,
Michael G. Rosenfeld
Publication year - 1995
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.92.7.2720
Subject(s) - lim domain , pou domain , homeobox , biology , anterior pituitary , transcription factor , microbiology and biotechnology , emx2 , pituitary gland , gene , genetics , zinc finger , endocrinology , hormone
A pituitary LIM homeodomain factor, P-Lim, is expressed as Rathke's pouch forms and as specific pituitary cell phenotypes are established, suggesting functional roles throughout pituitary development. While selectively expressed in both anterior and intermediate pituitary in mature mice, P-Lim is also transiently expressed in the developing ventral neural cord and brainstem. P-Lim binds to and activates the promoter of the alpha-glycoprotein subunit gene, a marker of early pituitary development, and synergizes with Pit-1 in transcriptional activation of genes encoding terminal differentiation markers. The LIM domain of P-Lim specifically interacts with the Pit-1 POU domain and is required for synergistic interactions with Pit-1, but not for basal transcriptional activation events.