Open AccessRep protein of tomato yellow leaf curl geminivirus has an ATPase activity required for viral DNA replication.
Author(s) -
Cécile Desbiez,
C. David,
Amel Mettouchi,
Jürgen Laufs,
Bruno Gronenborn
Publication year - 1995
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.92.12.5640
Subject(s) - biology , geminiviridae , tomato yellow leaf curl virus , viral replication , dna replication , ter protein , mutant , dna , peptide sequence , atpase , microbiology and biotechnology , replication factor c , amino acid , virology , plant virus , virus , gene , biochemistry , origin of replication , control of chromosome duplication , enzyme , begomovirus
The Rep protein of geminiviruses is the sole viral protein required for their DNA replication. The amino acid sequence of Rep protein contains an NTP binding consensus motif (P-loop). Here we show that purified Rep protein of tomato yellow leaf curl virus expressed in Escherichia coli exhibits an ATPase activity in vitro. Amino acid exchanges in the P-loop sequence of Rep causes a substantial decrease or loss of the ATPase activity. In vivo, mutant viruses carrying these Rep mutations do not replicate in plant cells. These results show that ATP binding by the Rep protein of geminiviruses is required for its function in viral DNA replication.