Open AccessA secondary-structure model for the self-cleaving region of Neurospora VS RNA.
Author(s) -
Tara L. Beattie,
Joan E. Olive,
Richard A. Collins
Publication year - 1995
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.92.10.4686
Subject(s) - rna , ribozyme , protein secondary structure , ligase ribozyme , nucleic acid structure , neurospora , chemistry , pseudoknot , nucleic acid secondary structure , hairpin ribozyme , biochemistry , cleavage (geology) , biology , stereochemistry , gene , neurospora crassa , paleontology , fracture (geology) , mutant
Neurospora VS RNA performs an RNA-mediated self-cleavage reaction whose products contain 2',3'-cyclic phosphate and 5'-hydroxyl termini. This reaction is similar to those of hammerhead, hairpin, and hepatitis delta virus ribozymes; however, VS RNA is not similar in sequence to these other self-cleaving motifs. Here we propose a model for the secondary structure of the self-cleaving region of VS RNA, supported by site-directed mutagenesis and chemical modification structure probing data. The secondary structure of VS RNA is distinct from those of the other naturally occurring RNA self-cleaving domains. In addition to a unique secondary structure, several Mg-dependent interactions occur during the folding of VS RNA into its active tertiary conformation.