Open AccessPP1 gamma 2, a testis-specific protein-serine/threonine-phosphatase type 1 catalytic subunit, is associated with a protein having high sequence homology with the 78-kDa glucose-regulated protein, a member of the 70-kDa heat shock protein family.
Author(s) -
Yang–Sook Chun,
Hiroshi Shima,
Koichi Nagasaki,
Takashi Sügimura,
Minako Nagao
Publication year - 1994
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.91.8.3319
Subject(s) - protein subunit , microbiology and biotechnology , biology , biochemistry , polyacrylamide gel electrophoresis , phosphatase , gel electrophoresis , threonine , protein phosphatase 1 , peptide sequence , serine , protein phosphatase 2 , enzyme , gene
Protein phosphatase 1 gamma 2 (PP1 gamma 2) is a testis-specific isotype of the protein-serine/threonine-phosphatase type 1 catalytic subunit. Three native forms of PP1 gamma 2 were detected in a crude fraction of rat testis by electrophoresis in a nondenaturing polyacrylamide gel. We purified a major native form of PP1 gamma 2 to homogeneity by successive column chromatography on Mono Q-Sepharose, EAH-agarose, protamine-agarose, and G3000SW and by electrophoresis in a nondenaturing polyacrylamide gel. The G3000SW-purified PP1 gamma 2 native form had an apparent molecular mass of 170 kDa. The purified holoenzyme from nondenaturing polyacrylamide gel was composed of the catalytic subunit and two noncatalytic subunits, of 78 kDa and 55 kDa. Partial amino acid sequence analysis of the 78-kDa protein suggested that it is the 78-kDa glucose-regulated protein, a member of the 70-kDa heat shock protein family. The 78-kDa protein may possibly function as a chaperone or by confining substrate specificity of PP1 gamma 2.