Open AccessNuclear matrix proteins in human coloncancer.
Author(s) -
Susan K. Keesee,
Marc D. Meneghini,
Robert P. Szaro,
Yuelong Wu
Publication year - 1994
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.91.5.1913
Subject(s) - nuclear matrix , nuclear protein , biology , matrix (chemical analysis) , pathology , colorectal cancer , adenocarcinoma , cell nucleus , chromatin , cancer , nucleus , microbiology and biotechnology , chemistry , gene , genetics , medicine , transcription factor , chromatography
The nuclear matrix is the nonchromatin scaffoldingof the nucleus. This structure confers nuclear shape, organizes chromatin, andappears to contain important regulatory proteins. Tissue specific nuclear matrixproteins have been found in the rat, mouse, and human. In this study we comparedhigh-resolution two-dimensional gel electropherograms of nuclear matrix proteinpatterns found in human colon tumors with those from normal colon epithelia.Tumors were obtained from 18 patients undergoing partial colectomy foradenocarcinoma of the colon and compared with tissue from 10 normal colons. Wehave identified at least six proteins which were present in 18 of 18 colontumors and 0 of 10 normal tissues, as well as four proteins present in 0 of 18tumors and in 10 of 10 normal tissues. These data, which corroborate similarfindings of cancer-specific nuclear matrix proteins in prostate and breast,suggest that nuclear matrix proteins may serve as important markers for at leastsome types of cancer.