Open AccessMultiple tyrosine protein kinases in rat hippocampalneurons: isolation of Ptk-3, a receptor expressed in proliferative zones of thedeveloping brain.
Author(s) -
Marina P. Sánchez,
Peter Tapley,
Shamsher S. Saini,
Bin He,
Daniel Pulido,
Mariano Barbacid
Publication year - 1994
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.91.5.1819
Subject(s) - receptor protein tyrosine kinases , biology , receptor tyrosine kinase , tyrosine kinase , protein tyrosine phosphatase , microbiology and biotechnology , proto oncogene tyrosine protein kinase src , tyrosine , sh3 domain , ror1 , kinase , platelet derived growth factor receptor , signal transduction , receptor , biochemistry , growth factor
Tyrosine protein kinases are likely to play animportant role in the maintenance and/or development of the nervous system. Inthis study we have used the PCR cloning technique to isolate sequences derivedfrom tyrosine kinase genes expressed in cultured hippocampal neurons obtainedfrom 17.5-day-old rat embryos. Nucleotide sequence analysis of 209 independentclones revealed sequences derived from 25 tyrosine kinases, of which twocorresponded to previously unreported genes. One of the PCR clones, ptk-2,belongs to the Jak family of cytoplasmic tyrosine kinases. The second clone,ptk-3, was derived from a gene encoding an additional class of tyrosine kinasereceptors whose extracellular domains contain regions of homology withcoagulation factors V and VIII and complement component C1. Transcripts encodingthe Ptk-3 receptor are present in a variety of embryonic and adult tissues withhighest levels observed in brain. During development, ptk-3 transcripts are mostabundant in the proliferative neuroepithelial cells of the ventricular zone,raising the possibility that this receptor may play an important role in thegeneration of the mammalian nervous system.