Open AccessHuman immunodeficiency virus type 1 Nef associateswith a cellular serine kinase in T lymphocytes.
Author(s) -
Earl T. Sawai,
Andreas S. Baur,
H Struble,
B. Matija Peterlin,
Jay A. Levy,
Cecilia ChengMayer
Publication year - 1994
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.91.4.1539
Subject(s) - serine , kinase , protein kinase a , biology , map2k7 , c raf , virology , cyclin dependent kinase 9 , mitogen activated protein kinase kinase , cyclin dependent kinase 2 , phosphorylation , microbiology and biotechnology , biochemistry
With T-cell lines constitutively expressing Neffrom the SF2 strain of human immunodeficiency virus type 1 (HIV-1SF2) in theform of a hybrid CD8-Nef fusion protein or T-cell lines chronically infectedwith HIV-1SF2, a cellular serine kinase was found that specifically associateswith Nef. Proteins of 62 kDa and 72 kDa, which coimmunoprecipitated with Nef,were phosphorylated in in vitro kinase assays. This Nef-associated serine kinaseactivity was not blocked by inhibitors of protein kinase C or protein kinase Aand was lost when Nef was truncated at amino acid 94 or 99. These findingspresent evidence that a serine kinase activity is associated with Nef expressedin human T lymphocytes.