Open AccessIn vivo transcription from the adenovirus E2 earlypromoter by RNA polymerase III.
Author(s) -
Wenlin Huang,
Ronald Pruzan,
S. J. Flint
Publication year - 1994
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.91.4.1265
Subject(s) - rna polymerase i , microbiology and biotechnology , polymerase , rna polymerase iii , rna dependent rna polymerase , rna polymerase ii , small nuclear rna , transcription factor ii d , transcription (linguistics) , biology , rna polymerase , rna , rnase h , rnase p , gene expression , gene , promoter , genetics , linguistics , philosophy
We have previously reported that the subgroup Cadenovirus E2 early (E2E) RNA polymerase II promoter can specify efficient invitro transcription by RNA polymerase III. We now show that promoter proximalsequences of the E2E transcription unit are also transcribed by RNA polymeraseIII in nuclei isolated from adenovirus-infected cells. Small E2E RNA speciesthat possessed the same properties as in vitro synthesized RNA polymerase IIIE2E transcripts were detected in cytoplasmic RNA populations from infected cellsby using blotting, primer extension, and RNase protection assays. The 3'termini of these RNAs were mapped to thymidine-rich sequences typical of RNApolymerase III termination sites. These results demonstrate that a single genecan be transcribed by both RNA polymerase II and RNA polymerase III invivo.