Open AccessA small element from the Mason-Pfizer monkey virusgenome makes human immunodeficiency virus type 1 expression and replicationRev-independent.
Author(s) -
Molly S. Bray,
Susan Prasad,
John W. Dubay,
Eric Hunter,
Kuan Teh Jeang,
David Rekosh,
Marie Louise Hammarskjöld
Publication year - 1994
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.91.4.1256
Subject(s) - subgenomic mrna , biology , retrovirus , untranslated region , virology , group specific antigen , virus , viral replication , three prime untranslated region , genetics , messenger rna , genome , gene
Replication of human immunodeficiency virus type 1(HIV-1) is dependent on the viral Rev protein. This protein acts in concert withthe cis-acting rev-responsive element present in intron-containing RNAs tofacilitate nuclear export of these RNAs. Here we show that a cis-acting219-nucleotide sequence from an unrelated "simple" retrovirus,Mason-Pfizer monkey virus (MPMV), enables Rev-independent HIV-1 replication.This sequence is present in an untranslated region near the 3' end of theMPMV genome. The MPMV element is also able to efficiently substitute for Rev inexpression of Gag/Pol and Env proteins from subgenomic constructs. Wehypothesize that the MPMV element functions by interacting with a cellularfactor that plays a role in mRNA transport analogous to that of the Rev protein.It might be possible to exploit this element in the development of an HIVvaccine.