Open AccessA truncated bone morphogenetic protein receptor affects dorsal-ventral patterning in the early Xenopus embryo.
Author(s) -
Atsushi Suzuki,
R. Scott Thies,
Noboru Yamaji,
Jun Tae Song,
John M. Wozney,
Kazuo Murakami,
Naoto Ueno
Publication year - 1994
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.91.22.10255
Subject(s) - xenopus , bone morphogenetic protein , biology , microbiology and biotechnology , mesoderm , bone morphogenetic protein receptor , embryo , genetics , embryonic stem cell , gene
Bone morphogenetic proteins (BMPs), which are members of the transforming growth factor beta (TGF-beta) superfamily, have been implicated in bone formation and the regulation of early development. To better understand the roles of BMPs in Xenopus laevis embryogenesis, we have cloned a cDNA coding for a serine/threonine kinase receptor that binds BMP-2 and BMP-4. To analyze its function, we attempted to block the BMP signaling pathway in Xenopus embryos by using a dominant-negative mutant of the BMP receptor. When the mutant receptor lacking the putative serine/threonine kinase domain was expressed in ventral blastomeres of Xenopus embryos, these blastomeres were respecified to dorsal mesoderm, eventually resulting in the formation of a secondary body axis. These findings suggest that endogenous BMP-2 and BMP-4 are involved in the dorsal-ventral specification in the embryo and that ventral fate requires induction rather than resulting from an absence of dorsal specification.