Open AccessCharacterization of the promoter region of the gene for the rat neutral and basic amino acid transporter and chromosomal localization of the human gene.
Author(s) -
Ning Yan,
R Mosckovitz,
Louise Gerber,
S Mathew,
V.V.V.S. Murty,
S S Tate,
Sidney Udenfriend
Publication year - 1994
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.91.16.7548
Subject(s) - gene , biology , primer extension , genetics , promoter , transcription (linguistics) , microbiology and biotechnology , gene mapping , primer (cosmetics) , chromosome , gene expression , chemistry , rna , linguistics , philosophy , organic chemistry
The promoter region of the rat kidney neutral and basic amino acid transporter (NBAT) gene has been isolated and sequenced. The major transcription initiation site was mapped by primer extension. The entire promoter region and a set of 5' deletions within it were expressed at a high level in LLC-PK1 cells using the luciferase indicator gene. Positive and negative regulatory elements in the promoter region were observed. A human genomic clone of the transporter was also obtained and was used to localize the NBAT gene at the p21 region of chromosome 2.