Open AccessMolecular and functional analysis of a lymphocyte chemoattractant factor: association of biologic function with CD4 expression.
Author(s) -
William W. Cruikshank,
Nauman Nisar,
Minjie Wu,
Barbara Natke,
Arthur C. Theodore,
Hardy Kornfeld
Publication year - 1994
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.91.11.5109
Subject(s) - lymphocyte , chemotaxis , biology , complementary dna , cloning (programming) , expression cloning , microbiology and biotechnology , molecular cloning , interleukin 2 , cytokine , receptor , function (biology) , immunology , gene , genetics , computer science , programming language
Lymphocyte chemoattractant factor (LCF) is a lymphocyte cell product that stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. In concert with its chemoattractant activity, LCF induces human T-lymphocyte expression of interleukin 2 receptor. Here we describe the molecular cloning of cDNA encoding human LCF. It is a novel interleukin with no significant homology to any previously described cytokine families. There is an absolute requirement for both autoaggregation of LCF monomers and for membrane-expressed CD4 molecules for LCF-induced migration in lymphocytes.