Spermidine deficiency increases +1 ribosomal frameshifting efficiency and inhibits Ty1 retrotransposition in Saccharomyces cerevisiae. | Zendy

David Balasundaram | Zendy; Jonathan D. Dinman | Zendy; Reed B. Wickner | Zendy; C W Tabor | Zendy; Herbert Tabor | Zendy

Open Access

Spermidine deficiency increases +1 ribosomal frameshifting efficiency and inhibits Ty1 retrotransposition in Saccharomyces cerevisiae.

Author(s) -

David Balasundaram,

Jonathan D. Dinman,

Reed B. Wickner,

C W Tabor,

Herbert Tabor

Publication year - 1994

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.91.1.172

Subject(s) - translational frameshift , retrotransposon , saccharomyces cerevisiae , biology , ribosome , pseudoknot , ribosomal rna , transfer rna , spermidine , translation (biology) , ribosomal protein , rna , genetics , mutant , yeast , gene , messenger rna , biochemistry , transposable element , enzyme

Polyamines have been implicated in nucleic acid-related functions and in protein biosynthesis. RNA sequences that specifically direct ribosomes to shift reading frame in the -1 and +1 directions may be used to probe the mechanisms controlling translational fidelity. We examined the effects of spermidine on translational fidelity by an in vivo assay in which changes in beta-galactosidase activity are dependent on yeast retrovirus Ty +1 and yeast double-stranded RNA virus L-A -1 ribosomal frameshifting signals. In spe2 delta mutants of Saccharomyces cerevisiae, which cannot make spermidine as a result of a deletion in the SPE2 gene, there is a marked elevation in +1 but no change in -1 ribosomal frameshifting. The increase in +1 ribosomal frameshifting efficiency is accompanied by a striking decrease in Ty1 retrotransposition.

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