Open AccessControlled release of polypeptides from polyanhydrides.
Author(s) -
Eyal Ron,
Thomas Turek,
Edith Mathiowitz,
Mark Chasin,
Michael J. Hageman,
Robert Langer
Publication year - 1993
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.90.9.4176
Subject(s) - excipient , chemistry , chromatography , polymer , recombinant dna , size exclusion chromatography , high performance liquid chromatography , radioimmunoassay , biochemistry , organic chemistry , gene , enzyme
The effects of both polymer hydrophobicity and addition of stabilizers on the release and integrity of polymer-encapsulated proteins were studied. By using very hydrophobic poly[1,3-bis(p-carboxyhydroxy)hexane anhydride] with sucrose as an excipient, both recombinant bovine somatotropin and zinc insulin were released intact over 3 weeks. The released proteins appeared to maintain their integrity as judged by acidic reverse-phase HPLC, size-exclusion HPLC, radioimmunoassay, and conformation-sensitive immunoassays. Our results also suggest how polymer hydrophobicity can be used to enhance protein stability.