Open AccessInterleukin 13, a T-cell-derived cytokine that regulates human monocyte and B-cell function.
Author(s) -
Andrew N. J. McKenzie,
Janice Culpepper,
René de Waal Malefyt,
Francine Brière,
Juha Punn,
Gregorio Aversa,
Atsushi Sato,
W Dang,
Benjamin G. Cocks,
Satish Me
Publication year - 1993
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.90.8.3735
Subject(s) - biology , microbiology and biotechnology , complementary dna , immunoglobulin d , antibody , cdna library , t cell , b cell , cd23 , clone (java method) , immunoglobulin e , immunology , gene , immune system , biochemistry
We have isolated the human cDNA homologue of a mouse helper T-cell-specific cDNA sequence, called P600, from an activated human T-cell cDNA library. The human cDNA encodes a secreted, mainly unglycosylated, protein with a relative molecular mass of approximately 10,000. We show that the human and mouse proteins cause extensive morphological changes to human monocytes with an associated up-regulation of major histocompatibility complex class II antigens and the low-affinity receptor for immunoglobulin E (Fc epsilon RII or CD23). In addition, they stimulate proliferation of human B cells that have been activated by anti-IgM antibodies or by anti-CD40 monoclonal antibodies presented by a mouse Ltk- cell line transfected with CDw32. Furthermore, the human protein induced considerable levels of IgM and IgG, but no IgA production, in cultures in which highly purified human surface IgD+ or total B cells were cocultured with an activated CD4+ T-cell clone. Based on these findings, we propose that this immunoregulatory protein be designated interleukin 13.