Open AccessData base analysis of protein expression patterns during T-cell ontogeny and activation.
Author(s) -
Samir Hanash,
John R. Strahler,
Yishin Chan,
R. Kuick,
Daniel Teichroew,
James V. Neel,
Nabil Hailat,
David R. Keim,
Jeanne Gratiot-Deans,
David Ungar,
R. Melhem,
Xiaoxiang Zhu,
P. Andrews,
F. Lottspeich,
Christoph Eckerskorn,
Ernest H. Y. Chu,
Imran Ali,
David A. Fox,
Bruce C. Richardson,
Laurence A. Turka
Publication year - 1993
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.90.8.3314
Subject(s) - biology , mitosis , microbiology and biotechnology , gel electrophoresis , t cell , ontogeny , immunology , genetics , immune system
We have developed a data base of lymphoid proteins detectable by two-dimensional polyacrylamide gel electrophoresis. The data base contains two-dimensional patterns and derived information pertaining to polypeptide constituents of unstimulated and stimulated mature T cells and immature thymocytes, single-cell-derived T- and B-cell clones, leukemia cells, and lymphoid cell lines. Using this data base, we have compared the protein constituents of mature T cells and immature thymocytes before and after mitotic stimulation. A subset of polypeptides that are induced in mature T cells following mitotic stimulation were found to be constitutively expressed in immature thymocytes. Other polypeptides exhibited differences in their expression between mature and immature thymocytes in a manner unrelated to proliferation. The identity of several constitutively expressed or mitotically induced proteins in lymphoid cells was established by microsequencing. These initial findings point to significant differences in the molecular pathways leading to proliferation between mature and immature T cells. The construction of this database should facilitate further studies of lymphoid differentiation and function.