Open AccessGenomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein.
Author(s) -
Satoshi Inoue,
Akira Orimo,
Takayuki Hosoi,
Shigeru Kondo,
Hideo Toyoshima,
Takashi Kondo,
Akira Ikegami,
Yasuyoshi Ouchi,
Hajime Orimo,
Masami Muramatsu
Publication year - 1993
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.90.23.11117
Subject(s) - enhancer , zinc finger , estrogen receptor , hormone response element , biology , estrogen receptor alpha , estrogen receptor beta , transcription factor , microbiology and biotechnology , estrogen , untranslated region , gene , genetics , messenger rna , cancer , breast cancer
Estrogen receptor (ER)-binding fragments were isolated from human genomic DNA by using a recombinant ER protein. Using one of these fragments as a probe, we have identified an estrogen-responsive gene that encodes a putative zinc finger protein. It has a RING finger motif present in a family of apparent DNA-binding proteins and is designated estrogen-responsive finger protein (efp). efp cDNA contains a consensus estrogen-responsive element at the 3' untranslated region that can act as a downstream estrogen-dependent enhancer. Moreover, efp is regulated by estrogen as demonstrated at both the mRNA and the protein level in ER-positive cells derived from mammary gland. These data suggest that efp may represent an estrogen-responsive transcription factor that mediates phenotypic expression of the diverse estrogen action. Thus, the genomic binding-site cloning may be applicable for isolation of the target genes of other transcription factors.