Open AccessTranscription factors are required for the meiotic recombination hotspot at the HIS4 locus in Saccharomyces cerevisiae.
Author(s) -
Michael A. White,
Margaret Dominska,
Thomas D. Petes
Publication year - 1993
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.90.14.6621
Subject(s) - saccharomyces cerevisiae , recombination , flp frt recombination , genetics , transcription (linguistics) , meiosis , chromatin , biology , genetic recombination , eukaryotic transcription , homologous recombination , dna , microbiology and biotechnology , gene , promoter , gene expression , linguistics , philosophy
The full activity of a recombination initiation site located 5' of HIS4 requires the binding of the transcription factors RAP1, BAS1, and BAS2. Two RAP1 binding sites can substitute for the wild-type initiation site. A 51-bp region of telomeric DNA inserted upstream of either HIS4 or ARG4 very strongly stimulates recombination. We suggest that the ability of transcription factors to induce recombination is a consequence of an altered chromatin structure that favors the entry of proteins that initiate recombination, rather than an effect of these factors on transcription.