Open AccessDirect selection of antibodies that coordinate metals from semisynthetic combinatorial libraries.
Author(s) -
Carlos F. Barbas,
Jonathan S. Rosenblum,
Richard A. Lerner
Publication year - 1993
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.90.14.6385
Subject(s) - reagent , combinatorial chemistry , selection (genetic algorithm) , recombinant dna , chemistry , catalysis , metal , metal ions in aqueous solution , biochemistry , computer science , organic chemistry , machine learning , gene
An iterative strategy for the selection of catalytic metalloantibodies is described. The first stage of this strategy is validated by the selection of semisynthetic antibodies that coordinate a variety of different metal ions and the metal oxide magnetite. These results have implications not only for the development of catalytic metalloantibodies but also for the development of reagents for magnetic resonance imaging, delivery of radioisotopes, and purification of recombinant proteins.