Open AccessSix mRNAs with different 5' ends are encoded by a single gamma-glutamyltransferase gene in mouse.
Author(s) -
Sridharan Rajagopalan,
Da Fang Wan,
Geetha M. Habib,
Antonia R. Sepulveda,
Michael R. McLeod,
Russell M. Lebovitz,
Michael W. Lieberman
Publication year - 1993
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.90.13.6179
Subject(s) - biology , gene , microbiology and biotechnology , complementary dna , rna splicing , gene expression , rna , genetics , alternative splicing , promoter , exon
The 5' region of the mouse gamma-glutamyltransferase (gamma GT; EC 2.3.2.2) gene has been cloned and analyzed. This analysis, combined with sequence information obtained from gamma GT cDNA clones, indicates that in mouse a single gamma GT gene codes for six different mRNAs that differ in their 5' sequences. Analysis of steady-state levels of gamma GT RNA reveals different expression patterns for these RNAs in different organs. The six different 5' sequences are widely separated within a 10-kb region and three of them show 75-86% identify with the three known rat gamma GT cDNAs. Although the heterogeneity of the 5' ends of gamma GT RNAs may be explained in part by alternative splicing, it is likely that multiple promoters are involved in their generation.