Open AccessConserved sequence-tagged sites: a phylogenetic approach to genome mapping.
Author(s) -
Richard Mazzarella,
V. Montanaro,
Juha Kere,
Rolland Reinbold,
Alfredo Ciccodicola,
Michele D’Urso,
David Schlessinger
Publication year - 1992
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.89.9.3681
Subject(s) - biology , genome , phylogenetic tree , genetics , computational biology , primer (cosmetics) , sequence (biology) , human genome , conserved sequence , gene , dna sequencing , phylogenetics , evolutionary biology , base sequence , chemistry , organic chemistry
Cognate sites in genomes that diverged approximately 100 million years ago can be detected by PCR assays based on primer pairs from unique sequences. The great majority of such syntenically equivalent sequence-tagged sites (STSs) from human DNA can be used to assemble and format corresponding maps for other primates, and some based on gene sequences are shown to be useful for mouse and rat as well. Universal genomic mapping strategies may be possible by using sets of STSs common to many mammalian species.